Cryopreservation and Transport of Mouse Spermatozoa at -79.DEG.C..
نویسندگان
چکیده
منابع مشابه
Cryopreservation of spermatozoa: a 1996 review.
Both the ability to freeze human spermatozoa and the possibility of pregnancy following intrauterine insemination have existed for >40 years. There have been a number of improvements during that time concerning the methods of freezing and thawing human spermatozoa. Initially, the use of the cryoprotective properties of glycerol allowed a major improvement; subsequently, changes were mainly empi...
متن کاملSpermatozoa retrieval for cryopreservation after death
OBJECTIVES To describe the retrieval spermatozoa technique for cryopreservation after death, including the proximal part of vas deferens. MATERIAL AND METHODS A 28-years old man, with previous history of infertility, who died 12 hours ago, was submitted to spermatozoa retrieval for cryopreservation, with surgical bilateral resection in bloc of the proximal part of vas deferens, testicle and e...
متن کاملComparison of cryoprotectants and methods of cryopreservation of fowl spermatozoa.
The deleterious effects of three cryoprotectants, glycerol, dimethylsulfoxide (DMSO), and dimethylacetamide (DMA), were compared on fowl spermatozoa. The viability and integrity of spermatozoa were measured with eosin-nigrosin smears. Glycerol was the least deleterious cryoprotectant, followed by DMA, and DMSO was the most toxic. Methods employing either glycerol or DMA were then compared for t...
متن کاملCryopreservation of spermatozoa from freeze-tolerant and -intolerant anurans.
Spermatozoa of the freeze-tolerant wood frog (Rana sylvatica) were used to develop a general protocol for the frozen storage of amphibian spermatozoa. Tolerance of spermatozoa to cryoprotective agents and freezing in suspension (-80 degrees C) was determined from rates of sperm lysis and dual-fluorochrome vital dye assays. We tested the efficacy of four cryoprotectants (Me2SO, methanol, glycero...
متن کاملImpact of Cryopreservation Process on Viability, Nitric Oxide and DNA Apoptosis in Fertile Human Spermatozoa
Introduction: Infertility is a common phenomenon in modern societies. Today, use of assisted reproductive technologies (ARTs) for treatment of infertility is common, and cryopreservation is one of these technologies. Cryopreservation has impact on the function and percentage of fertility of human sperm. In this study, motility, viability, nitric oxide, and DNA apoptosis were assessed before and...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 2001
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.50.83